Figure 3
From: Metal-free imidazolium salts inhibit the growth of hepatocellular carcinoma in a mouse model
IBN-1 and IBN-9, but not DPIM, suppressed the expression of survivin and Cdk4 in HCC cell lines. Both IBN-1 and IBN-9 (10 and 100 μM, 24 h) inhibited the protein expression of survivin and Cdk4 (a) and Cyclin D1 (b) in the HLE cells. Similarly IBN-1 and IBN-9 (10 and 100 μM, 24 h) also inhibited the protein expression of survivin (c) in the Huh7 cells. At a slightly higher concentration (160 μM, 24 h), IBN-9 not only suppressed Cdk4, but also its binding partner Cyclin Ds and their downstream targets pBR and E2F1 (d), which would help to arrest cells in the G1-phase. Finally, DPIM did not suppress survivin nor any Cdks in both cell lines.
