Figure 4
From: Metal-free imidazolium salts inhibit the growth of hepatocellular carcinoma in a mouse model
Effects of IMSs on survivin cellular translocation and assembly during mitosis. (a) Survivin locates in both nucleus (arrows) and cytoplasm (dotted arrows) in the HLE cells. An intense staining for survivin was seen in a cell undergoing through mitosis at the telophase (arrowhead). (b) Treatment with 1000 μM of IBN-1 for 24 h translocated survivin from the nucleus (arrows) to the cytoplasm, presumably de-coupling the survivin–Cdk4 complex and preventing cells from entering the cycling process. (c) Survivin associated with α-tubulin in the anaphase (empty arrowheads) and telophase (arrowheads) is visible in the proliferating Huh7 cells, as revealed by survivin antibody. (d) Treatment with 200 μM of IBN-9 for 24 h completely abolished the survivin assembly and interfered with cell mitosis. (e, f) Images of (c, d) co-stained with DAPI. Note in (e) that the survivin assembly at the telophase (arrowheads) was located in the midbody between two newly formed nucleus in the adjacent daughter cells.
