Figure 6
Transcripts whose expression levels are altered by vesicular overexpressed in cancer prosurvival protein 1 (VOPP1) knockdown at 48 h post-small interfering (si)RNA transfection are enriched for annotations pertaining to oxidative stress. (a) Heat map of the 280 genes identified as differentially expressed 48 h after VOPP1 knockdown in the squamous cell carcinoma (SCC)-9 cell line. Genes (columns) were cluster by Euclidean distance and unweighted pair group method with arithmetic mean (UPGMA). The western blot confirms VOPP1 protein level knockdown from the same biological samples assessed by microarray (b) The Ingenuity® Systems pathway analysis tool results are presented. A list of the top 5 annotations enriched in the set of differentially expressed genes with VOPP1 knockdown from (a). The black vertical dashed line marks P=0.05, with the asterisks marking significant (P<0.05) results. Oxidative stress and mitochondrial dysfunction were identified as the most significantly altered gene expression annotations.
