Figure 4 | Laboratory Investigation

Figure 4

From: Inhibition of active autophagy induces apoptosis and increases chemosensitivity in cholangiocarcinoma

Figure 4

AdSiRNA targeting autophagy gene beclin 1-induced ICC cell arrest and apoptosis during nutrient starvation. (a, b) AdSi-beclin decreased beclin 1 mRNA and protein expression. (c) Deletion beclin 1 expression-induced G2/M phase arrest of QBC cells. Twelve hours after the virus infection, the cell medium was changed to EBSS incubating for another 48 h, and then the adherent cells were collected by trypsinization and determined with flow cytometry. Three individual experiments were performed, and the cell distribution in the cell cycle was determined by standard fluorescence-activated cell sorter analysis. The cell population in G2/M phase was shown. The x and y axes represent DNA content and the cell number, respectively. (d) Deletion of beclin 1 promoted cell apoptosis by starvation culture. QBC cells were infected with AdSi-beclin or AdSi-blank for 12 h, then the supernatant were changed for EBSS or normal medium incubating for another 48 h. Treated cells were stained and apoptosis was assessed by flow cytometry using an AnnexinV-FITC apoptosis detection kit.

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