Figure 4

miR-1 and -206 regulates CCND2. (a) mRNA microarray data represented as heatmap showing significant upregulation of CCND2 transcript in both alveolar (ARMS) and embryonal (ERMS) tumor tissue compared with normal skeletal muscle. (b) Sequence predictions shows potential binding sites for miR-1 and -206 in the 3′UTR of CCND2. (c) Quantification of CCND2 protein levels by western blot in JR1 and Rh30 cells transfected with either miR-1 or -206 precursor. Negative miRNA precursor served as control.