Figure 6
Cell function assays in JR1 cells ectopically expressing miR-29 a, b and c. (a) Cell cycle assay in JR1 cells transfected with 10 nM each of miR-29a or b or c. Compared with cells transfected with control precursor (I), miR-29a (II), -29b (III) or -29c (IV) transfected cells shows significant G1 arrest. Percentages of cells in each cell cycle phase are presented in (IV). (b) Cell cycle assays in JR1 cells transfected with miR-29 family members in combination (10 nM for each) shows significant G1 arrest (II) compared with control precursor transfected cells (I), and data are shown in (III). (c) Cell proliferation assays was conducted in JR1 cells 48 h after transfection with 10 nM miR-29 a or b or c, or all of the miR-29 family member together using CellTiter 96 Aqueous One Solution. Compared with control precursor transfected cells, JR1 cells transfected with miR-29 family members individually or in combinations shows significant inhibited cell proliferation. *P<0.05. (d) Vybrant apoptosis assays in serum-starved JR1 cells transfected with 10 nM miR-29 a or b or c. The assay was conducted 48 hours after transfection and miR-29a (II), -29b (III), or -29c (IV) transfected cells showed increased apoptosis compared with control cells (I), and data are presented in (V). (e) Apoptosis assays in serum-starved JR1 cells transfected with miR-29 family member in combination (10 nM for each) show pro-apoptosis (II) compared with control cells (I), data are shown in (III).
