Table 2 Serum levels and liver mRNA levels (RT-PCR) of inflammatory cytokines and liver MPO activity in control animals vs animals with steatosis

From: Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture

 

Controls (n=5)

Steatosis (n=5)

P-value

Serum IFN-γ (pg/ml)

51.12±40.63

37.58±15.04

0.505

Serum TNF-α (pg/ml)

14.47±4.85

21.20±8.07

0.245

Serum IL-1β (pg/ml)

36.88±14.04

49.72±17.01

0.229

Serum IL-6 (pg/ml)

29.13±12.63

29.42±33.52

0.986

ΔCt TNF-α

15.350±0.712

15.488±0.351

0.708

ΔCt IL-1β

7.878±1.023

7.816±0.500

0.906

ΔCt IL-6

16.695±0.682

17.178±0.608

0.298

Liver MPO (U/g liver tissue)

0.55±0.23

0.91±0.64

0.278

  1. Abbreviations: IFN-γ, interferon-gamma; TNF-α, tumour necrosis factor-α; IL-1β, interleukin-1β; IL-6, interleukin-6; MPO, myeloperoxidase.
  2. The mean values±s.d. of serum levels and liver tissue mRNA (expressed by ΔCt values) of IFN-γ, TNF-α, IL-1β and IL-6, as well as liver MPO activity are shown for control rats and rats with steatosis, respectively. The P-values of the comparison between rats on the control diet and rats on the methionine–choline-deficient diet are listed (independent samples t-test). A P-value <0.05 is considered statistically significant (*).
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