Table 3 Parameters of NO, TXA2 and ET-1 in control animals vs animals with steatosis

From: Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture

 

Controls (n=5)

Steatosis (n=5)

P-value

Serum NO (μmol/l)

26.76±5.34

32.04±6.34

0.236

Serum ET-1 (pg/ml)

14.39±2.03

18.48±1.36

0.006*

WB P-eNOS

0.0066±0.0018

0.0062±0.0011

0.684

WB eNOS

0.3080±0.1086

0.2743±0.0558

0.600

WB P-eNOS/eNOS

0.0200±0.0055

0.0245±0.0053

0.281

WB TXAS

0.0520±0.0087

0.0713±0.0083

0.012*

WB ET-1

0.0759±0.0150

0.0976±0.0068

0.043*

WB ETA

0.0397±0.0156

0.0299±0.0172

0.372

WB ETB

0.0278±0.0182

0.0232±0.0124

0.653

  1. Abbreviations: NO, nitric oxide; ET-1, endothelin-1; WB, western blot; P-eNOS, phophorylated endothelial NO synthase; TXAS, thromboxane A2 synthase; ETA, endothelin receptor A; ETB, endothelin receptor B.
  2. The mean values±s.d. of serum levels of NO and ET-1, and of liver tissue. WB-normalized densitometry values for P-eNOS, NOS, TXAS, ET-1, ETA and ETB, and the ratio of P-eNOS/NOS WB densitometry values are shown for control rats and rats with steatosis, respectively. The P-values of the comparison between rats on the control diet and rats on the methionine–choline-deficient diet are listed (independent samples t-test). A P-value <0.05 is considered statistically significant (*).
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