Figure 5
From: Multicolored stain-free histopathology with coherent Raman imaging
Stimulated Raman scattering (SRS) label-free images of demyelination and stroke mouse models. (a) Two-color SRS image (green: CH2 image; blue: CH3-CH2 difference image) of a demyelinating lesion in a mouse with experimental allergic encephalomyelitis (EAE). Perivascular inflammatory cells and histiocytes with large lipid droplets from phagocytized myelin are visible. Red blood cells in a capillary are highlighted by arrows (hemoglobin image, red, not used in this picture). (b) Two-color SRS image of brain tissue in a mouse stroke model 3 days post stroke. Macrophages with many small lipid droplets are dispersed throughout the tissue. Dark areas represent edema fluid. (c, d) Hematoxylin & eosin-stained micrographs of (c) EAE and (d) stroke model from the same region. Scale bar, 25 μm.
