Figure 2 | Laboratory Investigation

Figure 2

From: Salvianolic acid B lowers portal pressure in cirrhotic rats and attenuates contraction of rat hepatic stellate cells by inhibiting RhoA signaling pathway

Figure 2

Sal B inhibits collagen lattice contraction by HSCs and decreases ET-1-induced MLC2 phosphorylation. (a) Time-dependent activation of rat HSCs in culture. Activated cells express α-SMA. Scale bar, 100 μm. (b) Appearance of collagen lattice at 48 h after dislodgement. (c) Graphic presentation shows the extent of contraction. Each experimental condition was carried out in triplicate and at least three independent experiments were performed. #P<0.01 versus control, *P<0.01 versus ET-1. (d–e) Cells were stimulated with ET-1 (10−8M) for various time periods, or pretreated with ML-7 (10−5 M), Y-27632 (10−5 M) or Sal B (10−5 M) for 30 min before ET-1 stimulation. The nonP-, monoP- and diP-MLC2 were separated on 10% glycerol gel and the identity of these bands was verified using anti-Ser19-MLC2 antibody (green). (f–g) The extent of MLC2 phosphorylation is calculated as mol PO4/mol MLC2. Data from three independent experiments, #P<0.01 versus control, *P<0.01 versus ET-1.

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