Figure 3
(a) Representative example of miR-205 ISH (red) performed on A431 cells transfected with the negative control miRNA inhibitor or miR-205 miRNA inhibitor merged with DAPI (blue). (b) Quantification of miR-205 knockdown by qISH from 24 random fields. (c) Quantification of miR-205 knockdown by qRT-PCR normalized by U6. (d) Representative example of miR-205 ISH performed on Mel-501 cells transfected with the mimic negative control or miR-205 mimic. (e) Quantification of miR-205 overexpression by qISH from 24 random fields. (f) Quantification of miR-205 overexpression by qRT-PCR normalized by U6. Scale bars represent 25 μm.
