Figure 2

Increased HSPA5 in human gastric cancer (GC) and intestinal metaplasia (IM) is independent of MIST1 expression and colocalizes with alcianophilia in IM. (a) Normal human stomach showing low to scanty level of HSPA5 at bases of gastric units lacking alcianophilia. (b) IM in H. pylori-positive GC patient exhibits overexpression of HSPA5 co-localizing with alcianophilia. (c) The top right corner of the tissue exhibits areas of dysplasia with IM to the bottom left. In dysplasia, increased HSPA5 did not co-localize with alcianophilia. (d) Co-localization of increased HSPA5 with alcianophilia was evident in IM in H. pylori-positive non-GC patient. An area of AB-positive IM (bottom, inset) with upregulation of HSPA5 is bordered by areas of PAS-positive normal gastric glands (top, inset). (e) HSPA5 expression (red) localizes to chief cell compartment at the bottom of normal gastric glands with occasional cells staining positive for both MIST1 (brown) and HSPA5 (arrow). (f) In H. pylori-induced gastric metaplasia and dysplasia, increased HSPA5 (brown) is accompanied by loss of MIST1 (blue). Occasional plasma cells positive for MIST1 expression are identified in the lamina propria of the dysplastic region (arrow).