Figure 6
From: Steatosis does not impair liver regeneration after partial hepatectomy

Western blot analysis of Erk1/2 phosphorylation and measurement of ATP in steatotic liver tissue before and after 70% hepatectomy. 70% hepatectomy (PHx) causes dephosphorylation of Erk1/2 in regenerating liver tissue in standard diet (SD)-fed mice. The majority of western diet (WD)-fed animals exhibited converse development with enhanced Erk1/2 phosphorylation postoperatively. Quantification of bands detected by phosphoErk1/2 antibody normalized to β-actin bands on PHx+1 (a), PHx+2 (b) and PHx+7 (c). White bars represent mice fed a standard diet; black bars refer to animals receiving WD. Representative western blot showing two individual mice each, fed SD or WD (d). (Quantification shown of n=4 animals per group as relative intensity of bands normalized to β-actin±s.e.m.). ATP levels are depicted as RLU/mg liver tissue and did not show significant differences between SD and WD liver tissue.