Figure 2

Effects of sorafenib on Ras-Raf and phosphoinositide 3-kinase-protein kinase B (PI3K-Akt) signal pathways. (a) Huh7 and (b) LH86 cells were treated with sorafenib as indicated. Cell lysates were prepared and subjected to western blotting to detect phosphorylated Raf (p-c-Raf), cleaved (c)-Raf, K-Ras (Kirsten rat sarcoma viral oncogene homolog), p473-Akt, and Akt with specific antibodies, respectively. β-Actin was used as an equal protein loading control. (c) Huh7 and (d) LH86 cells were treated with various concentrations of sorafenib for 24 h. Cell lysates were prepared and subjected to western blotting to detect p-c-Raf, c-Raf, K-Ras, p473-Akt, and Akt with specific antibodies, respectively. β-Actin was used as an equal protein loading control.