Figure 8
From: OPA1 downregulation is involved in sorafenib-induced apoptosis in hepatocellular carcinoma
Optic atrophy 1 (OPA1) expression in human normal liver tissues and liver tumor (hepatocellular carcinoma (HCC)) tissues. (a) Expression of OPA1, mitofusin 1 (Mfn1), and mitofusin 2 (Mfn2) proteins in adjacent normal tissues and malignant liver tissues. Lysates from adjacent normal liver tissues and liver tumor tissues from different patients were prepared and subjected to western blotting. OPA1, Mfn1, and Mfn2 expression levels were detected with specific antibodies. β-Actin protein levels were assessed as an equal protein loading control (P1–12: randomly selected patient number). (b) OPA1 protein level analysis in paired normal liver (NL) tissues and liver tumor (HCC) tissues, and paired cirrhotic liver tissues and liver tumor tissues (HCC). Relative band intensities (RBIs) of OPA1 were analyzed by the Image J software to show relative OPA1 protein levels in paired tissues from patients. Statistical analysis was performed to show average OPA1 expression level in different matched tissue groups (*P<0.05).
