Figure 5 | Laboratory Investigation

Figure 5

From: Carbon tetrachloride-induced hepatic injury through formation of oxidized diacylglycerol and activation of the PKC/NF-κB pathway

Figure 5

Physical association of protein kinase C α (PKCα) and nuclear factor (NF)-κB p65 subunit. The livers from vehicle-administered (Cont.) and carbon tetrachloride-treated rats harvested 2, 6, 24, 48 and 72 h after administration were homogenized and subjected to immunoprecipitation using isoform-specific anti-PKC antibodies. Left panel, the supernatant fraction (Sup) were analyzed by immunoblotting with antibodies against phosphorylated (p-NF-κB p65), non-phosphorylated NF-κB p65 subunit (NF-κB p65) or PKC isoforms. The β-actin bands were used to correct for protein loading. Right panels, the liver homogenates were immunoprecipitated (IP) with antibodies against PKC isoforms, and the precipitates were analyzed by immunoblotting using phosphorylated (p-NF-κB p65) or non-phosphorylated NF-κB p65 subunit (NF-κB p65) antibodies.

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