Figure 4
Effects of AGE-BSA and KCa3.1 channels on cell migration in rat VSMCs. (a) Mean values of migrated cell number determined with Boyden chamber technique in cells treated with 200 μg/ml BSA, 200 μg/ml AGE-BSA, AGE-BSA plus 100 nM TRAM-34, or 5 μg/ml anti-RAGE antibody for 24 h (n=6, **P<0.01 vs control or BSA, ##P<0.01 vs AGE-BSA alone). (b) Western blots of KCa3.1 channels in cells transfected with 100 nM control siRNA or KCa3.1 siRNA for 24 h. (c) Mean values of migrated cell number in cells transfected with control siRNA or KCa3.1 siRNA (100 nM) and treated with 200 μg/ml AGE-BSA or 200 μg/ml BSA (n=6, **P<0.01 vs control siRNA with BSA; ##P<0.01 vs control siRNA with AGE-BSA).
