Figure 5
From: Angiostatin production increases in response to decreased nitric oxide in aging rat kidney
Angiostatin generation in response to decreased nitric oxide in human umbilical vein endothelial cells. After treatment with L-NG-nitroarginine methyl ester (L-NAME) for 24 h, the medium was changed to acidic medium. After 1 h, human plasminogen was added, and the cells were incubated for 1 h. The medium was collected, and western blot analysis was performed for angiostatin. Pepstatin A (Pep A) was used as a cathepsin D (CTPD) inhibitor. Bovine CTPD was used as a positive control. n=4 for each group. Values are expressed as mean±s.e.m. *P<0.05 versus control. †P<0.05 versus 5 mM L-NAME.
