Figure 4

Gene expression in mouse livers after 12 weeks of normal diet (ND) or high-fat diet (HFD) consumption. (a) Expression of B-cell-activating factor receptor (BAFF-R), transmembrane activator, calcium modulator, cyclophilin ligand interactor (TACI), and B-cell maturation factor (BCMA) genes. The data are normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) in the same sample. (b–e) Expression of genes associated with uptake and transportation (b), biosynthesis and activation (c), oxidation, very low-density lipoprotein production, fibrosis (d), and inflammation (e). (f) Fatty acid synthase (FAS) protein expression in mouse livers after 12 weeks of ND or HFD consumption was analyzed by western blotting. Gene expression data were normalized to those of GAPDH and were expressed as a ratio to those obtained for the wild-type (WT) mice after 12 weeks of ND feeding (n=7 each group). Error bars in the graphs indicate means±s.e.m. *P<0.05, **P<0.01, and ***P<0.001. ACC, acetyl-CoA carboxylase; ACS, acetyl-CoA synthetase; Apo, apoprotein; FABP, fatty acid-binding protein; IL, interleukin; MCP-1, monocyte chemotactic protein-1; MTP, microsomal triglyceride transfer protein; PPAR, peroxisome proliferator-activated receptor; SCD, stearoyl-CoA desaturase; SREBP, sterol regulatory element-binding protein; TGF, transforming growth factor; TNF, tumor necrosis factor.