Figure 5 | Laboratory Investigation

Figure 5

From: Blockade of B-cell-activating factor signaling enhances hepatic steatosis induced by a high-fat diet and improves insulin sensitivity

Figure 5

Effects of B-cell-activating factor (BAFF) on hepatic lipogenesis in Hepa1-6 cells. (a) BAFF receptor (BAFF-R) protein expression in Hepa1-6 cells and primary murine hepatocytes were analyzed by western blotting. (b) Induction of nuclear factor-κB (NF-κB) in Hepa1-6 cells treated with BAFF (n=8 per group). (c) Changes in gene expression in Hepa1-6 cells treated with BAFF (n=10 per group). (d) Fatty acid synthase (FAS) mRNA expression in Hepa1-6 cells treated with BAFF and BAFF-R Fc. Gene expression data were normalized to those of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). (e) Lipid accumulation in Hepa1-6 cells exposed to oleic acid in the presence or absence of BAFF (n=8 per group). Error bars in the graphs indicate means±s.e.m. *P<0.05, **P<0.01, and ***P<0.001. ACC, acetyl-CoA carboxylase; ACS, acetyl-CoA synthetase; SCD, stearoyl-CoA desaturase; SREBP, sterol regulatory element-binding protein; TG, triglycerides.

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