Figure 3 | Laboratory Investigation

Figure 3

From: Alterations in Brca1 expression in mouse ovarian granulosa cells have short-term and long-term consequences on estrogen-responsive organs

Figure 3

Presence of osteoclasts in long bones and in co-cultures of splenocytes and primary osteoblasts. (a) The sub-epiphyseal region of femoral bone shown on the left was processed for histological sectioning and subjected to tartrate-resistant acid phosphatase (TRAP) assay staining protocol, followed by counterstaining with hematoxylin. (b) Splenocytes from wild-type and mutant mice were harvested, co-cultured on glass chamber slides with primary osteoblasts to induce their differentiation into osteoclasts and subjected to the TRAP assay staining protocol, followed by counterstaining with hematoxylin. Arrows: TRAP-positive osteoclasts. Scale bars: a: 50 μm; b: 500 μm.

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