Figure 5
Consequences of a heterozygous Brca1 mutation on expression of Aromatase and Hsd3-beta by granulosa cells. Mice carrying either zero (Brca1+/+), one (Brca1+/−), or two (Brca1−/−) mutant alleles of Brca1 in their ovarian granulosa cells were inoculated with 5 IU pregnant mare serum gonadotropin (PMSG) for either 24 (left panels) or 48 h (right panels). Five mice were used in each group. Measurements were performed in each individual mouse within each group, without attempting to identify and discard any outlier. The ovaries were collected, homogenized and total cellular proteins were extracted. Aromatase (top) and Hsd3-beta (bottom) protein levels were measured by quantitative western blot analyses as detailed in the methodology section. Each data point represents the average of three independent experiments, each using five different sets of mice in each group. Bars represent s.e.
