Figure 1 | Laboratory Investigation

Figure 1

From: Cytokines induce tight junction disassembly in airway cells via an EGFR-dependent MAPK/ERK1/2-pathway

Figure 1

Representative photomicrographs obtained by fluorescent microscopy after immunofluorescence staining of zonula occludens (ZO)-1 and occludin expression in Calu-3 cells under basal culture conditions (a–f) or after stimulation for 48 h with TNF-α, (g–i), IL-4 (l–n) or INF-γ (o–q). Red staining (top panels) represents ZO-1, green staining (middle panels) occludin and (bottom panels) merged images of both occludin and ZO-1, where areas of colocalization appear as yellow. Unstimulated cells (a–c) stained with normal mouse (for occludin) or rabbit (for ZO-1) non-specific immunoglobulins were used as negative control. Original magnification × 63. Each picture is representing one confocal layer. The results shown are representative of the results obtained in six independent experiments. Scale bar: 50 μm.

Back to article page