Figure 3 | Laboratory Investigation

Figure 3

From: Cytokines induce tight junction disassembly in airway cells via an EGFR-dependent MAPK/ERK1/2-pathway

Figure 3

Representative photomicrographs obtained by fluorescent microscopy after immunofluorescence staining of the analysis of DNA cleavage by terminal transferase deoxyuridine triphosphate nick-end labeling (TUNEL) assay in Calu-3 cultured in medium alone (Control), (a) with DNAse I (positive control) (b) or in the presence of TNF-α, (c) IL-4 (d) and INF-γ (e) for 48 h. Apoptotic nuclei are displayed by green fluorescence whereas viable DAPI counter-stained nuclei appeared blue. Original magnification × 40. The results shown are representative of five independent experiments. Scale bar: 25 μm.

Back to article page