Figure 4

The capacity of the cells to fill the scratched area was examined under microscopy at the indicated time points. Confluent cell monolayers were scraped by a pipette tip in the central area. The SCC9 cells transfected with a vector encoding human Snail (SCC9-S) cells did not close the wound after 48 h. The photographs were taken at × 40 magnification, and the scale bar=500 μm.