Figure 6

The overexpression of Snail generates a cancer stem cell (CSC)-like phenotype in SCC9 cells transfected with a vector encoding human Snail (SCC9-S) cells. The flow cytometry analysis of the CD24, CD44, and CD133 expression in the SCC9-S and SCC9 cells transfected with an empty vector (SCC9-N) cells. (a) The expression of CD24 was downregulated in the SCC9-S cells. (b) A high level of CD44 expression was assessed in the two cell lines. (c) CD133 expression was not detected in the two cell lines. Mouse immunoglobulins conjugated to PE or PE-CY5 were used as isotype controls in each experiment.