Figure 3 | Laboratory Investigation

Figure 3

From: Transcription factor Runx2 is a regulator of epithelial–mesenchymal transition and invasion in thyroid carcinomas

Figure 3

Regulation of Runx2 expression. (a) RT-PCR. Fetal bovine serum (FBS) stimulation increases RUNX2 mRNA in a concentration-dependent manner in WRO and TPC-1 cells, and MEK inhibitor (U0126) significantly suppressed RUNX2 expression in these two cell lines. (b) Real-time PCR quantitatively measures RUNX2 mRNA (*P<0.05 versus control (FBS 0%)). (c) Immunocytofluorescence labeling of Runx2 shows induction of Runx2 expression after serum stimulation in WRO and TPC-1 cells. Cellular nuclei are labeled with DAPI (small square). (d) Western blotting. Serum stimulation increases Runx2 protein expression in WRO and TPC-1 cells, whereas U0126 reduces the expression of Runx2 in both cell lines. U0126 entirely blocks ERK phosphorylation in WRO cells and partially blocks those in TPC-1 cells.

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