Table 1 PCR primers
Gene symbol | Gene accession | Primer sequence (5′–3′) | AT (°C) | Product size (bp) |
|---|---|---|---|---|
RUNX1 | NM_001754 | F: GAACCACTCCACTGCCTTTAAC | 58 | 193 |
R: AAGTTCTGCAGAGAGGGTTGTC | ||||
RUNX2 | NM_004348 | F: GACCAGTCTTACCCCTCCTACC | 58 | 190 |
R: CTGCCTGGCTCTTCTTACTGAG | ||||
RUNX3 | NM_004350 | F: AGGCAATGACGAGAACTACTCC | 58 | 150 |
R: GTGGGGTTGGTGAACACAGT | ||||
SNAI1 | NM_005985 | F: CCAATCGGAAGCCTAACTACAG | 58 | 155 |
R: GACAGAGTCCCAGATGAGCATT | ||||
SNAI2 | NM_003068 | F: ACAGCGAACTGGACACACATAC | 58 | 182 |
R: GTATCCGGAAAGAGGAGAGAGG | ||||
SNAI3 | NM_351083 | F: GGAGACGCAGAGAGAAATCAAT | 58 | 216 |
R: ACCTCGCTGACTTCCAAGG | ||||
TWIST1 | NM_000474 | F: CATCGACTTCCTCTACCAGGTC | 58 | 219 |
R: TCCATTTTCTCCTTCTCTGGAA | ||||
MMP1 | NM_002421 | F: CAGATTCTACATGCGCACAAAT | 58 | 137 |
R: CTTTGAAAAACCGGACTTCATC | ||||
MMP2 | NM_004530 | F: ACAAAGAGTTGGCAGTGCAATA | 58 | 138 |
R: TCTGGTCAAGATCACCTGTCTG | ||||
MMP9 | NM_004994 | F: CAGTCCACCCTTGTGCTCTT | 58 | 130 |
R: CCAGAGATTTCGACTCTCCAC | ||||
VEGFA | NM_003376 | F: TACATCTTCAAGCCATCCTGTG | 58 | 169 |
R: TGTTGTGCTGTAGGAAGCTCAT | ||||
VEGFC | NM_005429 | F: TGGCAACATAACAGAGAACAGG | 58 | 129 |
R: CATGCATTGAGTCTTTCTCCAC | ||||
PGK1 | NM_000291 | F: GCTGACAAGTTTGATGAGAAT | 58 | 359 |
R: AGGACTTTACCTTCCAGGAGC | ||||
GAPDH | NM_002046 | F: GATGACATCAAGAAGGTGGTGA | 58 | 186 |
R: TTCGTTGTCATACCAGGAAATG |
