Figure 2

QIA of Feulgen-stained liver. (a) Images (Feulgen) were analyzed as described in Materials and methods. The customized algorithm identified each hepatocyte nucleus in blue (Overlay) and calculated its size. Scale bar length is 50 μm. At least one fixative from each of the fixative classes was assessed for nuclear size (b), nuclei per unit area (c), and the ratio of nucleus to cytoplasm (d). Fixative classes are identified by color.