Figure 4 | Laboratory Investigation

Figure 4

From: Quantitation of fixative-induced morphologic and antigenic variation in mouse and human breast cancers

Figure 4

Tabular and graph representation of data: Conversion of tabular image analysis data of ER and PR from a single representative ER/PR-positive SSM2 tumor into density:intensity graphs. The raw data obtained from the image analysis include calculations of percentages and absolute numbers of positive and negative tumors cells, and the area scanned (a, c). The raw data indicate the number of events recorded and the magnitude of differences between fixatives. The data are then normalized by conversion into density of positive cells per unit area for each fixative. Graphs provide visual representation of the effects of the four fixatives on IHC for ER and PR (b, d).

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