Figure 4
Differentiation abilities of the new chondrosarcoma (CS) cell lines. CH03, CH56, CAL78 cells, and mesenchymal stem cells (MSCs) were cultured in standard, chondrogenic (both as 3D pellets), osteogenic or adipogenic (both as cell monolayer) medium. Their differentiation status was then determined by specific staining: Alcian blue on paraffin-embedded pellets for production of cartilaginous matrix by chondrocytic cells, alizarin red S for matrix mineralization by osteoblastic cells and nile red to reveal lipid droplets in adipocytic cells (original magnification × 20, × 10 and × 40, respectively). Chondro, chondrogenic differentiation; osteo, osteoblastic differentiation; adipo, adipogenic differentiation; N/A, not available, pellets spontaneously disintegrated.
