Figure 6
Chemosensitivity of chondrosarcoma (CS) cell lines. (a) HOS, A673, CH56, and CH03 cells in monolayer were treated with increasing concentrations of DOX, MAF, or CIS for 3 days. Viable cells were then quantified by XTT assay. All experiments were carried out at least three times in triplicate. Graphs show data from one representative experiment, error bars indicate s.d. (b) CH03 cells were cultured as 3D pellets in standard or chondrogenic medium and then treated with DOX, MAF, or CIS for 3 days. After dissociation, cells were cultured for 3 additional days as monolayer in standard medium (without drug) and viable cells were then quantified by trypan blue staining. In parallel, CH03 cells in monolayer were treated with the drugs for 3 days and viable cells were then quantified by trypan blue staining. Experiments with 3D pellets and cells in monolayers were performed twice. Results are expressed as the mean±s.d. of these two experimental data. CH03 pellets chondro, CH03 3D chondrogenic pellets. (c) CH56 cells were treated with DOX as in (b). CH56 pellets chondro, CH56 3D chondrogenic pellets. (d) CH03 cells were cultured as 3D pellets in standard or chondrogenic medium and then treated with DOX for 3 days. Accumulation of DOX was analyzed by fluorescence microscopy, nuclei were visualized after DAPI staining and cartilaginous matrix formation after Alcian blue staining (inset, original magnification × 20). Experiments were performed twice, one representative experiment is presented here. Chondro, chondrogenic 3D pellets; DOX, doxorubicin.
