Figure 1 | Laboratory Investigation

Figure 1

From: Specific transduction and labeling of pancreatic ducts by targeted recombinant viral infusion into mouse pancreatic ducts

Figure 1

(a) Schematic representation of the AAV plasmid constructs used. pAAV-CMV-ZsGreen is a well-established AAV construct.26, 27 The other rAAV plasmid vectors, pAAV-Sox9-ZsGreen, pAAV-Sox9-iCre, and pAAV-CMV-ZsGreen-Stop-Floxed-FarRed were constructed from pAAV-CMV-ZsGreen (see Materials and Methods for details). (b) Western Blotting for expression of ZsGreen protein and Cre recombinase from AAV6-Sox9-ZsGreen and AAV6-Sox9-cre virus, respectively. Protein was extracted from HEK293 cells infected with AAV6-Sox9-ZsGreen virus 10 μl of 1.8 × 1012 GCP/ml (lane 2) or 10 ul of 2.3 × 1012 GCP/ml with AAV6-Sox9-cre (lane 4), and non-infection control (lane 1 and 3); 42 kD β-actin was used as control (upper row). (c) Determination of GFP and cre expression in vitro. The HEK293 cells were infected with AAV6-Sox9-ZsGreen 48hr (bright view in A and green in B); the HEK293/ZsGreen-Lox-Stop-Lox-FarRed cells (C) were infected with AAV6-Sox9-cre 48 h and cre cleavage cells shows red fluorescence (D).

Back to article page