Figure 5 | Laboratory Investigation

Figure 5

From: Specific transduction and labeling of pancreatic ducts by targeted recombinant viral infusion into mouse pancreatic ducts

Figure 5Figure 5

Expression of sox9-cre in the pancreatic ducts of LSL-Tm mice. Cre-induced lineage-tagging (red fluorescence) was seen in the pancreatic ducts of LSL-Tm mouse pancreas 1 week after AAV6-Sox9-cre virus infusion (gross dissecting microscope images shown in panel a, fluorescent, and b, white light). However, the CMV promoter-driven cre was expressed in many non-ductal cells (gross dissecting microscope images shown in panels c, fluorescent, and d, white light). Whole-mount staining of DBA (green in panels e and h), and histological section staining of DBA (green in panels k, n, and q) show Sox9-cre-induced lineage-tagging specifically of LSL-Tm mouse pancreatic ducts (f, i, l, o and r), including main ducts (f and l), interlobular and intralobar ducts (i, o and r). The sections are counterstained with DAPI in the merged images (m, p and s). Insets in panels k and l demonstrate red fluorescent-labeled cells that are DBA-negative stained, but appear to be ductal as they are in the wall of the duct. Whole-mount staining of CMV promoter-driven cre virus-infused pancreas shows Cre recombination also occurred in acinar cells and islets (t, u, and v). In histologic sections, immunofluorescent staining and diffuse Cre-induced red fluorescence in the AAV6-CMV-Cre infused pancreas is shown (wy), insulin (light blue), DBA (green).

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