Figure 2
From: Blockade of Smad signaling by 3′-deoxyadenosine: a mechanism for its anti-fibrotic potential
Roles of adenosine receptors and adenosine transporter in the suppression of TGF-β and BMP-4 signaling by 3′-deoxyadenosine (3′-DA). (a, c) NRK-52E cells were exposed to TGF-β1 (a) or BMP-4 (c) in the absence or presence of 3′-DA, DPSPX (DPX; 10 nM), MRS1523 (MRS; 5 μM) or NBTI (10 μM) for 24 h and subjected to northern blot analysis of Col I and Col IV. The level of 28S ribosomal RNA is shown at the bottom as a loading control. Quantitative analysis of Col I normalized by the level of 28S is shown in graphs. (b, d) Cells transfected with p(CAGA)12-MLP-Luc (b) or pBRE-Luc (d) were treated with indicated agents for 24 h and subjected to luciferase assay. (e) Cells transfected with pBRE-Luc were treated with BMP-4 with or without Cl-IB-MECA (10 μM) for 24 h and subjected to chemiluminescent assay. Asterisks indicate statistically significant differences. NS, not statistically significant.
