Figure 3
From: Blockade of Smad signaling by 3′-deoxyadenosine: a mechanism for its anti-fibrotic potential
Reduction in the levels of Smad proteins by 3′-deoxyadenosine (3′-DA). (a, b) NRK-52E cells were pretreated with 3′-DA for indicated time periods, stimulated with or without TGF-β1 (a) or BMP-4 (b) for 1 h and subjected to western blot analysis of phosphorylated and total Smad proteins. The level of β-actin is shown at the bottom as a loading control. (c, d) SM43 cells were pretreated with 20 μg/ml 3′-DA for 8 h, stimulated with TGF-β1 (c) or BMP-4 (d) for 1 h and subjected to western blot analysis of Smad proteins. Quantification of individual signals normalized by the level of β-actin is presented as graphs. Asterisks indicate statistically significant differences.
