Figure 1 | Laboratory Investigation

Figure 1

From: Inhibition of collagen I accumulation reduces glomerulosclerosis by a Hic-5-dependent mechanism in experimental diabetic nephropathy

Figure 1

Increases in glomerular collagen I expression in diabetic nephropathy are reduced by TG-2 inhibition. Unilateral nephrectomized male Wistar rats were subjected to streptozotocin-induced diabetes and subsequently treated with the TG-2 inhibitor NTU281 or vehicle. Tissue sections were immunostained for collagen I, and glomerular expression quantified on a 0–5+ scale by a blinded observer for 25 consecutive glomerular cross-sections. (a) Photomicrographs show typical sections for control, diabetic, and NTU281-treated kidneys ( × 400 magnification) at day 240. (b) Photomicrograph ( × 1000 magnification) showing distribution of collagen in diabetic glomerular cells. (c) Histogram detailing glomerular collagen I staining quantified on a 0–5+ scale by a blinded observer for 25 consecutive glomerular cross-sections. Significant differences are marked as *P=0.01 or **P<0.01.

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