Figure 3 | Laboratory Investigation

Figure 3

From: Inhibition of collagen I accumulation reduces glomerulosclerosis by a Hic-5-dependent mechanism in experimental diabetic nephropathy

Figure 3

Reduced glomerular Hic-5/collagen I expression in NTU281-treated diabetic kidneys is associated with reduced glomerular hypercellularity and mesangial apoptosis. (a, b) A total of 25 consecutive glomerular cross-sections of control, diabetic, and diabetic+NTU281 kidneys at 240 days after STZ were counted by a blinded observer for glomerular cell number in PAS-stained kidney sections. Examples are shown of apoptotic bodies visualized by PAS staining (c) and by staining with ApopTag (d). Significant differences are marked as *P<0.05) or **P<0.01). (c) Photomicrograph ( × 400 magnification) of PAS-stained glomerulus showing typical apoptotic body. (d) Photomicrograph ( × 400 magnification) of apoptotic body stained with ApopTag. Section is counterstained with hematoxylin.

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