Figure 11

Confocal dual immunofluorescence reaction of α-DG (green), β-DG (green), AQP4 (red), and myosin (green) in control (a, d, g), mdx (b, e, h), and PDN mdx (c, f, i) GC muscles. The control myofibers show a higher α/β DG green (a, d) and AQP4 (g) red fluorescence at sarcolemma compared with the mdx (b, e, h) faintly labeled myofibers. On the contrary, the PDN mdx fibers display a restoring of an intense fluorescence signal for α/β DG (c, f) and AQP4 (i). Scale bar: a–i, 25 μm.