Figure 4 | Laboratory Investigation

Figure 4

From: Effects of prednisolone on the dystrophin-associated proteins in the blood–brain barrier and skeletal muscle of dystrophic mdx mice

Figure 4

Confocal dual immunofluorescence reaction of FVIII (red) and desmin (green) and laminin in control (a, d), mdx (b, e) and PDN mdx (c, f) vessels, and morphometric analysis of desmin and laminin expression (g). Mdx red-labeled endothelial cells are poorly subtended by a light green fluorescence signal of desmin (b) and by discontinuous laminin fluorescence (e, arrow). Control (a, d) and PDN-treated (c, f) vessels show an intense and pointed desmin green signal (a, c) and a strong continuous laminin fluorescence (d, f) subtending the endothelial cells. Scale bar: a–c, 16, 6 μm; d–f, 10 μm. (g) Desmin and laminin expression show a lower value in mdx mice compared with control, while a significant increase in PDN-treated mice compared with the mdx animals is detected.

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