Figure 7

Confocal dual immunofluorescence reaction of AQP4 (red) and β-DG (green) in control (a–c), mdx (d–f), and PDN mdx (g–i) vessels. Control (a–c) and PDN mdx (g–i) vessels show an intense orange fluorescence signal of colocalization along the entire abluminal side, instead the mdx vessels show a reduced expression and separate AQP4 and β-DG signal for some tract of the abluminal surface (d–f, arrow). Scale bar: a–i, 25 μm.