Table 1 PCR primers
From: Epigenetic changes in renal genes dysregulated in mouse and rat models of type 1 diabetes
Species | Gene | Forward | Reverse |
|---|---|---|---|
Mouse | COX2 | 5′-TTAAGGCATCACAGTCCGAG-3′ | 5′-TTGAATGTGAAGTTGACCCG-3′ |
Mouse | CypA | 5′-GTGGTCTTTGGGAAGGTGAA-3′ | 5′-TTACAGGACATTGCGAGCAG-3′ |
Mouse | S100A4/FSP-1 | 5′-GGTGTAGTTTGCCAGTGGGGG-3′ | 5′-TGCGAAGAAGCCAGAGTAAGGC-3′ |
Mouse | MCP-1 | 5′-GAATGAAGGTGGCTGCTATG-3′ | 5′-AACCCAGAAACATCCAATTCTC-3′ |
Mouse | Vim | 5′-TTCTCAGCATCACGATGACC-3′ | 5′-CGTCTTTTGGGGTGTCAGTT-3′ |
Mouse | Lamc1 | 5′-GGAACCTTCCCACACGGGTC-3′ | 5′-CGGTGCTGATGCCCTCAAGT-3′ |
Mouse | β-actin | 5′-GTCCACCTTCCAGCAGATGT-3′ | 5′-AAGCCATGCCAATGTTGTCT-3′ |
Rat | COX2 | 5′-TGCAGAGTTGAAAGCCCTCT-3′ | 5′-CCCCAAAGATAGCATCTGGA-3′ |
Rat | CypA | 5′-GTGGTCTTTGGGAAGGTGAA-3′ | 5′-TACAGGGTATTGCGAGCAGA-3′ |
Rat | S100A4/FSP-1 | 5′-AGGACAGACGAAGCTGCATT-3′ | 5′-CTCACAGCCAACATGGAAGA-3′ |
Rat | MCP-1 | 5′-GTGCTGACCCCAATAAGGAA-3′ | 5′-TGCTGAAGTCCTTAGGGTTGA-3′ |
Rat | Vim | 5′-ACTTCTCAGCACCACGATGA-3′ | 5′-CTTTTGGGGGTGTCAGTTGT-3′ |
Rat | Lamc1 | 5′-CCTCCAGGTTGTGAATGTCC-3′ | 5′-CTCAACGAGATCGAAGGCTC-3′ |
Rat | β-actin | 5′-CTCTGAACCCTAAGGCCAAC-3′ | 5′-GACCAGAGGCATACAGGGAC-3′ |
