Table 1 Specific oligonucleotide primers for real-time polymerase chain reaction

From: Transforming growth factor-α activates pancreatic stellate cells and may be involved in matrix metalloproteinase-1 upregulation

Species

Genes

Primer sequences

Human

GAPDH

5′-GAAGGTGAAGGTCGGAGTC-3′, 5′-GAAGATGGTGATGGGATTTC-3′

 

α1(I) collagen

5′-TCCTGGTCCTGCTGGCAAAGAA-3′, 5′-CACGCTGTCCAGCAATACCTTGA-3′

 

TIMP-1

5′-CAGAAGTCAACCAGACCACCTTATACC-3′, 5′-CGGTTGTGGGACCTGTGGAAGTATC-3′

 

TIMP-2

5′-GAATCGGTGAGGTCCTGTCCTGA-3′, 5′-CCTGCACACAAGCCCGGATAAA-3′

 

TIMP-3

5′-GAGAGTCTCTGTGGCCTTAAGCTGG-3′, 5′-CTGGGAAGAGTTAGTGTCCAAGGG-3′

 

TIMP-4

5′-AGACCTCACAGGCTCAGTCG-3′, 5′-CATTCCTGCCAGTCAGCCTG-3′

 

MMP-1

5′-CTGCTTACGAATTTGCCGACAGAG-3′, 5′-GCAGCATCGATATGCTTCACAGTT-3′

 

MMP-2

5′-CTCCTGACATTGACCTTGGCACCG-3′, 5′-CGTCACAGTCCGCCAAATGAAC-3′

  1. Abbreviations: GAPDH, glyceraldehyde-3-phophate dehydrogenase; MMP, matrix metalloproteinase; TIMP, tissue inhibitor of metalloproteinase.