Figure 2 | Laboratory Investigation

Figure 2

From: Immune complexes activate human endothelium involving the cell-signaling HMGB1-RAGE axis in the pathogenesis of lupus vasculitis

Figure 2

Western immunoblot showing that HMGB1 is included in the prepared ICs. Detection of HMGB1 protein in the prepared IC samples and in the negative controls by western immunoblot analysis. Lanes: (1) control IgG alone; (2) lupus IgG alone; (3) supernatants of untreated U937 cells and control IgG; (4) supernatants of untreated U937 cells and lupus IgG; (5) supernatants of UV-treated U937 cells and control IgG; and (6) supernatants of UV-treated U937 cells and lupus IgG (ICs).

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