Figure 3
ICs upregulate RAGE expression, which can be partially blocked by HMGB1 A-box or Bay 117082 in human CRL-1730 cells. Human CRL-1730 cells were treated with ICs or negative controls as described in the Methods or pretreated with either HMGB1 A-box (10 μg/ml) or Bay 117082 (10−6 M) for 1 h before treatment with ICs. (a) After incubation for 6 h, RAGE mRNA expression was detected by quantitative real-time PCR. Results are presented as the fold increases as compared with unstimulated cells. Bars represent the means±s.e. #P<0.05 as compared with unstimulated cells (n=3), *P<0.05 as compared with IC-stimulated cells (n=3). (b) After incubation for 16 h, cell surface expression of RAGE protein was detected by flow cytometry. Results are presented as the fold increases as compared with unstimulated cells. Bars represent the means±s.e. #P<0.05 as compared with unstimulated cells (n=3), *P<0.05 as compared with IC-stimulated cells (n=3). UN: unstimulated CRL-1730 cells; NC1: IgG from healthy donors alone; NC2: lupus IgG alone; NC3: supernatants of untreated U937 cells, and IgG from healthy donors; NC4: supernatants of untreated U937 cells and lupus IgG; NC5: supernatants of UV-treated U937 cells, and IgG from healthy donors; A-box: HMGB1 A-box; BAY: Bay 117082.
