Figure 4 | Laboratory Investigation

Figure 4

From: Immune complexes activate human endothelium involving the cell-signaling HMGB1-RAGE axis in the pathogenesis of lupus vasculitis

Figure 4

ICs upregulate ICAM-1 and VCAM-1 expression, which can be partially blocked by HMGB1 A-box, sRAGE, Bay 117082 or by those agents used in combination in human CRL-1730 cells. Human CRL-1730 cells were pretreated with HMGB1 A-box (10 μg/ml), sRAGE (20 μg/ml), Bay 117082 (10−6 M) or the agents used in combination for 1 h before treatment with ICs. (a, b) After incubation for 6 h, ICAM-1 and VCAM-1 mRNA expression was detected by quantitative real-time PCR. Results are presented as the fold increases as compared with negative control cells. Bars represent the means±s.e. *P<0.05 as compared with ICs-stimulated cells (n=3). (c, d) After incubation for 16 h, cell surface ICAM-1 and VCAM-1 protein expression was detected by flow cytometry. Results are presented as fold increases as compared with negative control cells. Bars represent the means±s.e. *P<0.05 as compared with ICs-stimulated cells (n=3). Human CRL-1730 cells treated with supernatants of untreated U937 cells and IgG from healthy donors served as negative control. A-box, HMGB1 A-box; BAY, Bay 117082.

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