Figure 3 | Laboratory Investigation

Figure 3

From: M2-polarized tumor-associated macrophages promoted epithelial–mesenchymal transition in pancreatic cancer cells, partially through TLR4/IL-10 signaling pathway

Figure 3

Coculture (CC) with M2-polarized tumor-associated macrophages (TAMs) increased the expressions of mesenchymal markers but decreased epithelial markers in pancreatic cancer cells. Panc-1 and BxPC-3 cancer cell lines were indirectly cocultured with M2-polarized TAMs using a transwell system. After 24 h, the cancer cells were collected and analyzed. (a) Vimentin mRNA and (b) protein expression, as well as (c) Snail mRNA and (d) protein expression increased in the cell lines in CC. (e) E-cadherin mRNA and (f) protein expression decreased in the cell lines in coculture. The data represent at least three independent experiments. MC, monoculture. The data represent at least three independent experiments. *P<0.05 versus MC.

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