Figure 7 | Laboratory Investigation

Figure 7

From: M2-polarized tumor-associated macrophages promoted epithelial–mesenchymal transition in pancreatic cancer cells, partially through TLR4/IL-10 signaling pathway

Figure 7

TLR4/interleukin-10 (IL-10) signaling has an essential role in EMT of pancreatic cancer cells induced by M2-polarized tumor-associated macrophages (TAMs). (a, b) TLR4 mRNA and protein expression in M2-polarized TAMs increased after 24-h coculture (CC) with pancreatic cancer cells. (c, d) TLR4 mRNA and protein in M2-polarized TAMs significantly decreased after transfection of TLR4 siRNA. (e, f) Knockdown of TLR4, inhibition of TLR4/IL-10 resulted in significant inhibition E-cadherin downregulation. Conversely, activation of M2-polarized TAMs by lipopolysaccharide (LPS) induced a more profound reduction of E-cadherin, but increased vimentin and snail. MC, monoculture. The data represent at least three independent experiments. *P<0.05 versus MC in panel a, *P<0.05 versus baseline in panel c.

Back to article page