Figure 8 | Laboratory Investigation

Figure 8

From: M2-polarized tumor-associated macrophages promoted epithelial–mesenchymal transition in pancreatic cancer cells, partially through TLR4/IL-10 signaling pathway

Figure 8

TLR4/interleukin-10 (IL-10) signaling has an essential role in neoplastic characteristics of pancreatic cancer cells. In coculture (CC) with M2-polarized tumor-associated macrophages (TAMs), the pancreatic cell lines were treated with either TLR4 siRNA, neutralizing antibodies of TLR4 and IL-10, and lipopolysaccharide (LPS), respectively. After 24 h, proliferation, migration, and proteolytic activities were determined by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) (a), transwell (b), and zymography (c, d), respectively. MC, monoculture. The data represent at least three independent experiments. *P<0.05 versus MC, #P<0.05 versus CC.

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