Figure 2
Butyrate acid enhanced milk fat globule-epidermal growth factor 8 (MFG-E8) expression in cell lines. (a) NCI-H716 cells were treated with different concentrations of butyrate for 48 h. Using quantitative real-time polymerase chain reaction (PCR), the transcriptional levels of MFG-E8 were analyzed relative to that of glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Values are shown as the mean and standard deviation of results from more than three independent experiments. **P<0.01 vs control (non-stimulated cells). (b) Time-dependent mRNA expression of MFG-E8 in various cell lines after stimulation with 10 mM butyrate. The culture conditions for each cell line are described in Materials and Methods (no addition of phorbol 12-myristate 13-acetate (PMA)). All signals were standardized to the intensity of GAPDH. **P<0.01 vs control (non-stimulated cells). (c) Time-dependent production of MFG-E8 proteins in NCI-H716 cells treated with 10 mM butyrate acid. MFG-E8 contents in culture supernatants were measured using an enzyme-linked immunosorbent assay (ELISA). Error bars indicate the standard error of mean values obtained from more than three independent experiments. **P<0.01 vs Control (non-stimulated cells).
